LOCUS pIRES1hyg 5726 bp ds-DNA circular 12-JUN-2008 DEFINITION Cloning vector pIRES1hyg, complete plasmid sequence. ACCESSION U89672 VERSION U89672.1 GI:1899166 KEYWORDS bicistronic. SOURCE unidentified cloning vector ORGANISM unidentified cloning vector other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5726) AUTHORS Rees,S., Coote,J., Stables,J., Goodson,S., Harris,S. and Lee,M.G. TITLE Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein JOURNAL BioTechniques 20 (1), 102-104 (1996) PUBMED 8770413 REFERENCE 2 (bases 1 to 5726) AUTHORS Lou,Y. and Holtz,A. TITLE Direct Submission JOURNAL Submitted (14-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424- 8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers intron 938..1233 /note="synthetic intron" /label=synthetic intron /ApEinfo_fwdcolor=#ffc01c /ApEinfo_revcolor=#ffc01c CDS 1872..2906 /codon_start=1 /transl_table=11 /product="hygromycin B phosphotransferase" /protein_id="AAB49979.1" /db_xref="GI:1899167" /translation="MDRSGKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDV GGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVT LQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADP HVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVI DWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQ LYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNR RPSTRPRAKE" /label=hygromycin B phosphotransferase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 3232..3332 /label=BGH polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 gene 4730..5591 /gene="bla" /label=bla /ApEinfo_fwdcolor=#00ff6e /ApEinfo_revcolor=#00ff6e CDS complement(4730..5590) /EC_number="3.5.2.6" /function="ampicillin-resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAB49980.1" /db_xref="GI:1899168" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /label=beta-lactamase /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 880..880 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 1274..1860 /label=EMCV-R IRES /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 863..881 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 236..852 /label=CMV immediate early enhancer/promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 237..819 /label=CMV immediate early enhancer/promoter up to -13 fro /ApEinfo_fwdcolor=#ff9000 /ApEinfo_revcolor=#ff9000 misc_feature 833..833 /label=putative transcription (+1) start site? /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa atTAATACGA CTCACTATAG Ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttaattcgct gtctgcgagg 961 gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg 1021 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg 1081 gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc 1141 aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca 1201 caggtgtcca ctcccaggtc caactgcagg tcgatcgagc atgcatctag ggcggccgca 1261 ctagaggaat tcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg 1321 aataaggccg gtgtgtgttt gtctatatgt gattttccac catattgccg tcttttggca 1381 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc 1441 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag 1501 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg 1561 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac 1621 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa 1681 gcgtagtcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggaatctgat 1741 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa gctctaggcc 1801 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc 1861 ccgtaccaaa gatggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt 1921 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat 1981 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg 2041 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga 2101 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc 2161 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc 2221 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg 2281 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga 2341 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg 2401 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc 2461 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg 2521 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct 2581 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc 2641 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac 2701 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg 2761 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg 2821 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca 2881 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag 2941 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac 3001 gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg 3061 gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca cgattgtcat 3121 gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc 3181 cgattgggtg cagatctaga gctcgctgat cagcctcgac tgtgcctcta gTTGCCAGCC 3241 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 3301 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCattgtctg agtaggtgtc attctattct 3361 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 3421 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgagtgc 3481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 3541 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 3601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 3661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 3721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 3781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 3841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 3901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 3961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 4141 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 4201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 4261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 4321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 4381 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 4441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 4501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 4561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 4621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 4681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 4741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 4801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 4861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 4921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 4981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 5161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 5221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 5281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 5341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 5401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 5461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 5521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 5581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 5641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 5701 ttccccgaaa agtgccacct gacgtc //