LOCUS pMC_WT 7461 bp ds-DNA circular 07-JUL-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Vecteur:pAB00 digA?Â?rA?Â? NheI Insert: leader 5' gypsy (852 pb) PCR digA?Â?rA?Â? NheI COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers misc_feature 864..882 /label=T7_RNApol_promoter (-17 to +2) /ApEinfo_fwdcolor=#80ff00 /ApEinfo_revcolor=#ff0000 misc_feature 881..881 /label=T7_transcription_start (+1) /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff misc_feature 820..820 /label=putative transcription start (+1) /ApEinfo_fwdcolor=#ff11ff /ApEinfo_revcolor=#ff11ff misc_feature 232..842 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 842..842 /label=putative transcription start (+1), based on GI:4097 /ApEinfo_fwdcolor=#ff23ff /ApEinfo_revcolor=#ff23ff misc_feature 735..735 /label=transcription start (+1), based on GI:3175993 /ApEinfo_fwdcolor=#ff23ff /ApEinfo_revcolor=#ff23ff misc_feature 1764..4829 /label=LacZ /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green misc_feature 5007..5120 /label=BGH polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 5007..5019 /label=end of bovine growth hormone CDS GI:30794287 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 5102..5102 /label=possible last base of mRNA copied from DNA template /ApEinfo_fwdcolor=#ff14ff /ApEinfo_revcolor=#ff14ff misc_feature 5647..6320 /label=colE1 ori /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 923..1768 /label=gypsy_1-846 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan primer_bind complement(5300..5320) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 864..883 /label=T7 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green primer_bind complement(4964..4981) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green CDS 1863..1931 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_binding complement(5326..5348) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature complement(6463..7325) /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#ffff00 ORIGIN 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTAACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCa agcttgatGG 901 GCTCGATCCC CTCGAGCTAG CGgttcaaat cttgtgtcga aataaaccac agcttgctcc 961 ggctcattgc cgttaaacat cattgttctt atttacaatc aaatcgctat cgccacaagg 1021 ctagtgataa taactaaggg ggcgaagtca agccctccaa cctaatctcc ataaacagtg 1081 tctaagacga acctcagcga aagaaggaag atctctagac ctactggaaa taacataact 1141 ctggacctat tggaacttat ataattggcg cccaaccaac aatctgaacc caccaatcta 1201 atttaacaca ctttgtcagg cgacaaacag ggtagttaag ttagaaaagc atgtaagttt 1261 tacaagacac ttctttgacg caatcaagaa atttacgagt gaaaaaaaaa aaaaaaaaaa 1321 gttgtgtatc tggccacgta ataagtgtgc gttgaattta ttcgcaaaaa cattgcatat 1381 tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg catacttttt 1441 agagaaacca aataattttt tattgcatac ccgtttttaa taaaatacat tgcataccct 1501 cttttaataa aaaatattgc atactttgac gaaacaaatt ttcgttgcat acccaataaa 1561 agattattat attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa 1621 aaatattgca tacgttgacg aaacaaattt tcgttgcata cccaataaaa gattattata 1681 ttgcatacct tttcttgcca taccatttag ccgatcaatt gtgctcggca acagtatatt 1741 tgtggtgtgc caaccaacaa ccaatgagGC TAGCGGATCC CGTCGTTTTA CAACGTCGTG 1801 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 1861 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA 1921 ATGGCGAATG GCGCTTTGCC TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG 1981 AGTGCGATCT TCCTGAGGCC GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT 2041 ACGATGCGCC CATCTACACC AACGTAACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC 2101 CCACGGAGAA TCCGACGGGT TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC 2161 AGGAAGGCCA GACGCGAATT ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA 2221 ACGGGCGCTG GGTCGGTTAC GGCCAGGACA GTCGTTTGCC GTCTGAATTT GACCTGAGCG 2281 CATTTTTACG CGCCGGAGAA AACCGCCTCG CGGTGATGGT GCTGCGTTGG AGTGACGGCA 2341 GTTATCTGGA AGATCAGGAT ATGTGGCGGA TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC 2401 TGCATAAACC GACTACACAA ATCAGCGATT TCCATGTTGC CACTCGCTTT AATGATGATT 2461 TCAGCCGCGC TGTACTGGAG GCTGAAGTTC AGATGTGCGG CGAGTTGCGT GACTACCTAC 2521 GGGTAACAGT TTCTTTATGG CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG 2581 GCGGTGAAAT TATCGATGAG CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG 2641 TCGAAAACCC GAAACTGTGG AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC 2701 TGCACACCGC CGACGGCACG CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG 2761 TGCGGATTGA AAATGGTCTG CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA 2821 ACCGTCACGA GCATCATCCT CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG 2881 ATATCCTGCT GATGAAGCAG AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC 2941 ATCCGCTGTG GTACACGCTG TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA 3001 TTGAAACCCA CGGCATGGTG CCAATGAATC GTCTGACCGA TGATCCGCGC TGGCTACCGG 3061 CGATGAGCGA ACGCGTAACG CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA 3121 TCTGGTCGCT GGGGAATGAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA 3181 TCAAATCTGT CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG 3241 CCACCGATAT TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG 3301 TGCCGAAATG GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC 3361 TTTGCGAATA CGCCCACGCG ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG 3421 CGTTTCGTCA GTATCCCCGT TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC 3481 TGATTAAATA TGATGAAAAC GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA 3541 CGCCGAACGA TCGCCAGTTC TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC 3601 CAGCGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA 3661 CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCAATGCGA TAACGAGCTC CTGCACTGGA 3721 TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC 3781 AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC GGGCAACTCT 3841 GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC GGGCACATCA 3901 GCGCCTGGCA GCAGTGGCGT CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC 3961 ACGCCATCCC GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC 4021 GTTGGCAATT TAACCGCCAG TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC 4081 AACTGCTGAC GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG 4141 TAAGTGAAGC GACCCGCATT GACCCTAACG CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC 4201 ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT GATGCGGTGC 4261 TGATTACGAC CGCTACCGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC AGCCGGAAAA 4321 CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA GTGGCGAGCG 4381 ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG 4441 TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT GCCGCCTGTT 4501 TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTATACCCC GTACGTCTTC CCGAGCGAAA 4561 ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT 4621 TCCAGTTCAA CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC 4681 TGCTGGACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 4741 GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTCCA GCTGAGCGCC GGTCGCTCAA 4801 ATTACCAGTT GGTCTGGTGT CAAAAATAAT AATAACCGGG CAGGGGGGAT CCGTCGAAGA 4861 TCAGGTTATC GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG 4921 ACCCCACCTG TAGGTTTGGC AAGCTAGtct agaGGGCCCT ATTCTATAGT GTCACCTAAA 4981 TGCTAGAGCT CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 5041 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 5101 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 5161 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT 5221 GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCGAGGGGGA TCCCGTCGAC 5281 CTCGAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 5341 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 5401 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 5461 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 5521 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 5581 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 5641 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 5701 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 5761 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 5821 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 5881 GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 5941 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 6001 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 6061 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 6121 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 6181 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 6241 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 6301 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 6361 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 6421 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 6481 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 6541 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 6601 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 6661 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 6721 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 6781 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 6841 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 6901 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 6961 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 7021 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 7081 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 7141 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 7201 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 7261 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 7321 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 7381 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 7441 CGAAAAGTGC CACCTGACGT C //