LOCUS GAL_497_1222_CAT_I 11243 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >GAL/497-1222/CAT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 6015..6861 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6670..6861) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6897..7128 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6878..6895) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(9552..10234) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 10329..11189 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7669..7994 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(9164..9184) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 8030..8824 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(9190..9212) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 7208..7514 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8999..9129 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..5259 /label=Hiap2_497-1222 /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=cyan misc_feature 5266..5925 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gaggcttgtg ggtattgact tcccccaaaa 4561 gttgtcagct gaagtaattt agcccactta agtaaatact atgatgataa gctgtgtgaa 4621 cttagctttt aaatagtgtg accatatgaa ggttttaatt acttttgttt attggaataa 4681 aatgagattt tttgggttgt catgttaaag tgcttatagg gaaagaagcc tgcatataat 4741 tttttacctt gtggcataat cagtaattgg tctgttattc aggcttcata gcttgtaacc 4801 aaatataaat aaaaggcata atttaggtat tctatagttg cttagaattt tgttaatata 4861 aatctctgtg aaaaatcaag gagttttaat attttcagaa gtgcatccac ctttcagggc 4921 tttaagttag tattactcaa gattatgaac aaatagcact taggttacct gaaagagtta 4981 ctacaacccc aaagagttgt gttctaagta gtatcttggt aattcagaga gatactcatc 5041 ctacctgaat ataaactgag ataaatccag taaagaaagt gtagtaaatt ctacataaga 5101 gtctatcatt gatttctttt tgtggtaaaa atcttagttc atgtgaagaa atttcatgtg 5161 aatgttttag ctatcaaaca gtactgtcac ctactcatgc acaaaactgc ctcccaaaga 5221 cttttcccag gtccctcgta tcaaaacatt aagagtatac tcgagatgga gaaaaaaatc 5281 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 5341 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 5401 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 5461 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg 5521 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 5581 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 5641 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 5701 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 5761 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 5821 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt 5881 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 5941 tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg 6001 gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac 6061 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt 6121 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa 6181 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca 6241 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga 6301 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg 6361 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg 6421 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat 6481 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac 6541 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat 6601 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 6661 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 6721 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6841 caatgtatct tatcatgtct gcctagaggg ccctattcta tagtgtcacc taaatgctag 6901 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 6961 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 7021 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 7081 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 7141 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 7201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 7261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 7321 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg 7381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 7441 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 7501 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 7561 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 7621 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag 7681 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 7741 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 7801 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 7861 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 7921 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 7981 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 8041 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 8101 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 8161 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 8221 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 8281 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 8341 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 8401 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 8461 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 8521 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 8581 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 8641 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 8701 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 8761 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 8821 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 8881 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 8941 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 9001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 9061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 9121 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 9181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 9781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 9961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 10021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 10981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11221 tatcagggtt attgtctcat gag //