LOCUS GAL_870_1222_CAT_I 10870 bp ds-DNA linear 02-OCT-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 291..945 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink promoter 946..964 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 945..964 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 962..962 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 913..913 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 1101..4244 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1278..1346 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 5642..6488 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink polyA_signal complement(6297..6488) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 6524..6755 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink primer_bind complement(6505..6522) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(9179..9861) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 9956..10816 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 7296..7621 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(8791..8811) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7657..8451 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(8817..8839) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed rep_origin 6835..7141 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 8626..8756 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4534..4886 /label=Hiap2_870-1222 /ApEinfo_fwdcolor=#80ff00 /ApEinfo_revcolor=green misc_feature 4893..5552 /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green ORIGIN 1 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 61 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact 121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg 361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc 541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 901 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat 961 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg 1021 aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct 1081 aaagcaaaaa agaagtcacc atgtcgttta ctttgaccaa caagaacgtg attttcgttg 1141 ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 1201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1321 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1381 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact 1441 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 1501 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg 1561 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt 1621 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg 1681 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc 1741 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 1801 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc 1861 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt 1921 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg 1981 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca 2041 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 2101 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg 2161 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc 2221 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc 2281 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt 2341 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 2401 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc 2461 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc 2521 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg 2581 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg 2641 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 2701 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga 2761 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg 2821 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact 2881 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg 2941 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 3001 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc 3061 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg 3121 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc 3181 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga 3241 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 3301 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc 3361 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg 3421 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga 3481 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg 3541 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 3601 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac 3661 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat 3721 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg 3781 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc 3841 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 3901 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg 3961 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc 4021 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa 4081 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc 4141 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 4201 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc 4261 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact 4321 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt 4381 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt 4441 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 4501 aactcggcct cgactctagg cggccgcctc gagccacctt tcagggcttt aagttagtat 4561 tactcaagat tatgaacaaa tagcacttag gttacctgaa agagttacta caaccccaaa 4621 gagttgtgtt ctaagtagta tcttggtaat tcagagagat actcatccta cctgaatata 4681 aactgagata aatccagtaa agaaagtgta gtaaattcta cataagagtc tatcattgat 4741 ttctttttgt ggtaaaaatc ttagttcatg tgaagaaatt tcatgtgaat gttttagcta 4801 tcaaacagta ctgtcaccta ctcatgcaca aaactgcctc ccaaagactt ttcccaggtc 4861 cctcgtatca aaacattaag agtatactcg agatggagaa aaaaatcact ggatatacca 4921 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 4981 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 5041 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 5101 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 5161 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 5221 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 5281 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 5341 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 5401 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 5461 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 5521 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt 5581 aaacgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc 5641 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag 5701 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga 5761 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt 5821 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg 5881 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc 5941 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa 6001 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa 6061 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac 6121 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat 6181 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg 6241 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 6301 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 6361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 6421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6481 catgtctgcc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca 6541 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 6601 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 6661 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 6721 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 6781 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 6841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 6901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 6961 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 7021 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 7081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 7141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 7201 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 7261 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 7321 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 7381 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 7441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7501 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 7561 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 7621 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 7681 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 7741 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 7801 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 7861 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 7921 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 7981 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 8041 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 8101 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 8161 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 8221 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 8281 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 8341 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 8401 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 8461 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 8521 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 8581 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 8641 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 8701 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 8761 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 8821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 9001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 9061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 9121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 9181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 9241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9361 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 9421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 9481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 9541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 9661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 10021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 10081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 10141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 10201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 10261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 10321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 10381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 10441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 10501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 10561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 10621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10861 gtctcatgag //