LOCUS pBgalutrophinACAT 11022 bp ds-DNA circular 02-SEP-2009 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 6500..6669 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 5823..6499 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T(1) /ApEinfo_label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink promoter 320..974 /vntifkey="29" /note="CMV promotor" /label=CMV\Promotor /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin complement(9360..10042) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 promoter 975..993 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 7016..7322 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 polyA_signal 6705..6936 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink promoter 7477..7802 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 1130..4273 /label=LacZ /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green CDS 1307..1375 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 10137..10997 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 974..993 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(8998..9020) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed 5'UTR 4562..5067 /vntifkey="52" /label=utrophin\A\IRES /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(6686..6703) /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 991..991 /label=T7 transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green polyA_signal complement(6500..6669) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal complement(6478..6499) /label=SV40 late polyA(1) /ApEinfo_label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 942..942 /label=CMV transcriptional start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green CDS 5074..5733 /vntifkey="4" /label=CAT /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#c0c0c0 misc_feature 7838..8632 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 8807..8937 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink primer_bind complement(8972..8992) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 61 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 121 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt 181 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa 241 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta 301 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga 361 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 421 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 481 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 541 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 721 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 781 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 841 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 901 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact 961 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga 1021 tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc 1081 gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa gaagtcacca tgtcgtttac 1141 tttgaccaac aagaacgtga ttttcgttgc cggtctggga ggcattggtc tggacaccag 1201 caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg 1261 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 1321 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 1381 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga 1441 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta 1501 caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac 1561 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg 1621 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg 1681 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg 1741 agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca 1801 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac 1861 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact 1921 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt 1981 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga 2041 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact 2101 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg 2161 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg 2221 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca 2281 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa 2341 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac 2401 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat 2461 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt 2521 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa 2581 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc 2641 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg 2701 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat 2761 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca 2821 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc 2881 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga 2941 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca 3001 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc 3061 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag 3121 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg 3181 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat 3241 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt 3301 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg 3361 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct 3421 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg 3481 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct 3541 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg 3601 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc 3661 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca 3721 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg 3781 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc 3841 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt 3901 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct 3961 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg 4021 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag 4081 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga 4141 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag 4201 cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg 4261 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc 4321 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta 4381 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca 4441 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt 4501 ccaaccgctg tttggtctgc tttctgacaa actcggcctc gactctaggc ggccgctcga 4561 gttgtggagt cgcccttccc ccgaggaggg gagcgggcag cgccgggcgc gggatttctc 4621 ccgcccaggg ccgaggagga gccctgggct cctcaaccga tcgggccacc tccgggagcg 4681 gcgccccttt tctttcgggt cattcctgca gagggaaaac tcctgcgtgc gcctgccccc 4741 aggctagcgt ttggactgtt ttgtttttgg cggaactacc gggcaggaag attgcacaag 4801 tcagggggcg tttccaatcg ggtgtcaatt ttggatctcc ccccccccac ccccctttgg 4861 atcttgtcgg gctttccacg tttcacttaa aaaaaattct tgtaacgtcc aaggcaagct 4921 ccccacgacc cgaacacgcc cagaggaacc ttggggcctc tggggacttg gctagcaggt 4981 attcatgcta gcctggacca tttttcagat ttagccttca gaagaggatc tgggaaagcc 5041 tttggattat actgaaactc attcaagctc gagatggaga aaaaaatcac tggatatacc 5101 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct 5161 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag 5221 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct 5281 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac 5341 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac 5401 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa 5461 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc 5521 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc 5581 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt 5641 caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa 5701 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct 5761 taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcgc 5821 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca 5881 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg 5941 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg 6001 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat 6061 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac 6121 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga 6181 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa 6241 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata 6301 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa 6361 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt 6421 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 6481 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 6541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6661 tcatgtctgc ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc 6721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 6781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 6841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 6901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 6961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 7021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 7081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 7141 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 7201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 7261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 7321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 7381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 7441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 7501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 7681 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 7741 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 7801 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 7861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 7921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 7981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 8041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 8101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 8161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 8221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 8281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 8341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 8401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 8461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 8521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 8581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 8641 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 8701 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 8761 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 8821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 8881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 8941 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 9001 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 9061 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 9121 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 9181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 9241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 9301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 9361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 9421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 9481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 9541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 9601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 9661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 9721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 9781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 9841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 9901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 9961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 10021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 10081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 10141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 10201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 10261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 10321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 10381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 10441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 10501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 10561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 10621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 10681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 10741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 10801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 10861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 10921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 10981 aaatgttgaa tactcatact cttccttttt caatattatt ga //