LOCUS deletion_1_31 6581 bp ds-DNA linear 30-JUN-2008 DEFINITION ACCESSION KEYWORDS SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 6612) AUTHORS Self JOURNAL Unpublished. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 3178..3837 /gene="CAT" /label=CAT /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#d0abd6 CDS 4828..6480 /gene="FFLuc" /label=FFLuc /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#d0abd6 primer_bind complement(24..41) /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#00ffff primer_bind 3078..3098 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 3104..3129 /label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 2028..2710 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS 112..180 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_binding 3050..3072 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed CDS 1271..1930 /label=beta lactamase /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow CDS 3178..3834 /label=CmR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature complement(183..636) /label=f1 ori /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#ff0080 CDS 3952..4820 /gene="BVDV" /label=BVDV /ApEinfo_fwdcolor=#0000a0 /ApEinfo_revcolor=#d0abd6 misc_feature 3128..3128 /label=SP6 transcription start /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green ORIGIN 1 TATAGTGAGT CGTATTACAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 61 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 121 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 181 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT 241 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA 301 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA 361 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT 421 AATCAAGTTT TTTGCGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC 481 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 541 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA 601 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCTG ATGCGGTATT TTCTCCTTAC 661 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC 721 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG 781 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA 841 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT 901 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 961 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 1021 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 1081 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 1141 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 1201 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 1261 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 1321 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 1381 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 1441 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 1501 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 1561 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 1621 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 1681 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 1741 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 1801 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 1861 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 1921 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 1981 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 2041 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 2101 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 2161 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 2221 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 2281 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 2341 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 2401 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 2461 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 2521 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 2581 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 2641 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 2701 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 2761 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC 2821 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC 2881 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG 2941 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA 3001 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG 3061 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TATTTAGGTG 3121 ACACTATAGA ATACTCAAGC TTATGCATGC GGCCGCTCAG GAGCTAAGGA AGCTAAAATG 3181 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT 3241 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT 3301 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC 3361 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG 3421 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG 3481 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG 3541 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT 3601 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC 3661 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC GCAAGGCGAC 3721 AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TCTGTGATGG CTTCCATGTC 3781 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT 3841 TTTTAAGGCA GTTATTGGTG CCCTTAAACG CCTGGTTCGT ACGCCTGAAT AAGTGATAAT 3901 AAGCGGATGA ATGGCAGAAA TTCGAGCGGC CGCATCTAGA GGGCCCGGAT CCGTATTGGG 3961 CAATTAAAAA TAATAATTAG GCCTAGGGAA CAAATCCCTC TCAGCGAAGG CCGAAAAGAG 4021 GCTAGCCATG CCCTTAGTAG GACTAGCATA ATGAGGGGGG TAGCAACAGT GGTGAGTTCG 4081 TTGGATGGCT TAAGCCCTGA GTACAGGGTA GTCGTCAGTG GTTCGACGCC TTGGAATAAA 4141 GGTCTCGAGA TGCCACGTGG ACGAGGGCAT GCCCAAAGCA CATCTTAACC TGAGCGGGGG 4201 TCGCCCAGGT AAAAGCAGTT TTAACCGACT GTTACGAATA CAGCCTGATA GGGTGCTGCA 4261 GAGGCCCACT GTATTGCTAC TAAAAATCTC TGCTGTACAT GGCACATGGA GTTGATCACA 4321 AATGAACTTT TATACAAAAC ATACAAACAA AAACCCGTCG GGGTGGAGGA ACCTGTTTAT 4381 GATCAGGCAG GTGATCCCTT ATTTGGTGAA AGGGGAGCAG TCCACCCTCA ATCGACGCTA 4441 AAGCTCCCAC ACAAGAGAGG GGAACGCGAT GTTCCAACCA ACTTGGCATC CTTACCAAAA 4501 AGAGGTGACT GCAGGTCGGG TAATAGCAGA GGACCTGTGA GCGGGATCTA CCTGAAGCCA 4561 GGGCCACTAT TTTACCAGGA CTATAAAGGT CCCGTCTATC ACAGGGCCCC GCTGGAGCTC 4621 TTTGAGGAGG GATCCATGTG TGAAACGACT AAACGGATAG GGAGAGTAAC TGGAAGTGAC 4681 GGAAAGCTGT ACCACATTTA TGTGTGTATA GATGGATGTA TAATAATAAA AAGTGCCACG 4741 AGAAGTTACC AAAGGGTGTT CAGGTGGGTC CATAATAGGC TTGACTGCCC TCTATGGGTC 4801 ACAACTTGCT CAGACACGAA GATCCAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG 4861 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA GGCTATGAAG 4921 AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG CACATATCGA GGTGAACATC 4981 ACGTACGCGG AATACTTCGA AATGTCCGTT CGGTTGGCAG AAGCTATGAA ACGATATGGG 5041 CTGAATACAA ATCACAGAAT CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG 5101 GTGTTGGGCG CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA 5161 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT TTCCAAAAAG 5221 GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA TAATCCAGAA AATTATTATC 5281 ATGGATTCTA AAACGGATTA CCAGGGATTT CAGTCGATGT ACACGTTCGT CACATCTCAT 5341 CTACCTCCCG GTTTTAATGA ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA 5401 ATTGCACTGA TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG 5461 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG CAATCAAATC 5521 ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC ACGGTTTTGG AATGTTTACT 5581 ACACTCGGAT ATTTGATATG TGGATTTCGA GTCGTCTTAA TGTATAGATT TGAAGAAGAG 5641 CTGTTTTTAC GATCCCTTCA GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA 5701 TTTTCATTCT TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA 5761 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC AAAACGCTTC 5821 CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA CTACATCAGC TATTCTGATT 5881 ACACCCGAGG GGGATGATAA ACCGGGCGCG GTCGGTAAAG TTGTTCCATT TTTTGAAGCG 5941 AAGGTTGTGG ATCTGGATAC CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT 6001 GTCAGAGGAC CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG 6061 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA AGACGAACAC 6121 TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA AAGGATATCA GGTGGCCCCC 6181 GCTGAATTGG AATCGATATT GTTACAACAC CCCAACATCT TCGACGCGGG CGTGGCAGGT 6241 CTTCCCGACG ATGACGCCGG TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG 6301 ACGATGACGG AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG 6361 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG AAAACTCGAC 6421 GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG GCGGAAAGTC CAAATTGTAA 6481 AATGTAACTG TATTCAGCGA TGACGAAATT CTTAGCTATT GTAATCCTCC GAGGCCTCGA 6541 GGTCGACGAA TTCGAGCTCG GCCGACTTGG CCAATTCGCC C //