LOCUS p_CMVbgalutrophinA 10341 bp ds-DNA circular 08-JUL-2008 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 23..41 /vntifkey="30" /note="T7 promotor" /label=T7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 6525..6850 /vntifkey="29" /note="SV40 promotor" /label=SV40 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(5734..5751) /vntifkey="21" /label=SP6 /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#ffff80 misc_feature 6838..7632 /label=Kan/Neo /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 7650..7667 /vntifkey="21" /note="Neomycin resistance gene" /label=Neo /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink 5'UTR 3562..4067 /vntifkey="52" /label=utrophin\A\IRES /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 307..375 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed polyA_signal complement(5478..5669) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 130..3273 /label=LacZ /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(7998..8020) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature 4871..5714 /vntifkey="21" /note=" SV40 small T antigen " /label=SV40_SM_T /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink misc_feature 4074..4733 /label=CAT /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=green polyA_signal 5753..5984 /vntifkey="25" /note="BGH poly A" /label=BGH\polyA /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=pink misc_feature 9137..9997 /label=beta lactamase /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(5686..5703) /label=SP6(1) /ApEinfo_label=SP6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 6016..6322 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 7855..7985 /vntifkey="21" /note="SV40 poly A" /label=SV40\polyA /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=pink primer_bind complement(7972..7992) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(8360..9042) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 ORIGIN 1 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attctgcaga 61 tatccatcac actggcggcc gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa 121 gaagtcacca tgtcgtttac tttgaccaac aagaacgtga ttttcgttgc cggtctggga 181 ggcattggtc tggacaccag caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt 241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 361 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 421 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 481 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt 541 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 601 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 661 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 721 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac 781 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 841 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 901 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 961 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 1021 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 1081 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 1141 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1201 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1261 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1321 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1381 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1441 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1501 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1561 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1621 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1681 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1741 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1801 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1861 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1921 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1981 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 2041 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 2101 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 2161 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2221 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2281 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2341 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2401 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2461 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2521 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2581 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2641 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2701 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2761 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2821 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2881 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2941 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 3001 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 3061 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 3121 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3181 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc 3241 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc 3301 gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc 3361 ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt tttcccgatt 3421 tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt tgccgctatt 3481 tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcggcctc 3541 gactctaggc ggccgctcga gttgtggagt cgcccttccc ccgaggaggg gagcgggcag 3601 cgccgggcgc gggatttctc ccgcccaggg ccgaggagga gccctgggct cctcaaccga 3661 tcgggccacc tccgggagcg gcgccccttt tctttcgggt cattcctgca gagggaaaac 3721 tcctgcgtgc gcctgccccc aggctagcgt ttggactgtt ttgtttttgg cggaactacc 3781 gggcaggaag attgcacaag tcagggggcg tttccaatcg ggtgtcaatt ttggatctcc 3841 ccccccccac ccccctttgg atcttgtcgg gctttccacg tttcacttaa aaaaaattct 3901 tgtaacgtcc aaggcaagct ccccacgacc cgaacacgcc cagaggaacc ttggggcctc 3961 tggggacttg gctagcaggt attcatgcta gcctggacca tttttcagat ttagccttca 4021 gaagaggatc tgggaaagcc tttggattat actgaaactc attcaagctc gagatggaga 4081 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 4141 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 4201 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 4261 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 4321 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 4381 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 4441 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 4501 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 4561 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 4621 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca 4681 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt 4741 aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg 4801 gatgaatggc agaaattcgc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat 4861 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg 4921 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa 4981 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag 5041 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa 5101 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc 5161 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag 5221 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata 5281 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta 5341 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata 5401 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta 5461 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 5521 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5641 aaactcatca atgtatctta tcatgtctgc ctagagggcc ctattctata gtgtcaccta 5701 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 5761 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 5821 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 5881 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 5941 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 6001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 6061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 6121 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 6181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 6241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 6301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 6361 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 6421 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 6481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 6541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 6601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 6661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 6721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 6781 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 6841 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 6901 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 6961 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 7021 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 7081 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 7141 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 7201 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 7261 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 7321 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 7381 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 7441 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 7501 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 7561 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 7621 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 7681 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 7741 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 7801 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 7861 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 7921 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 7981 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 8041 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 8101 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 8161 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 8221 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 8281 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 8341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 8401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 8461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 8521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 8581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 8641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 8701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 8761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 8821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 8881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 8941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9001 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 9061 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 9121 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 9181 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 9241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 9301 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 9361 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9421 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 9481 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 9541 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 9601 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 9661 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 9721 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 9781 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 9841 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 9901 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 9961 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 10021 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 10081 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 10141 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata 10201 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 10261 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 10321 taggcgtttt gcgctgcttc g //