LOCUS pEGFP-CAT-NDST2 6200 bp ds-DNA circular SYN 14-JUN-2007 DEFINITION . ACCESSION . VERSION . SOURCE . ORGANISM . . FEATURES Location/Qualifiers misc_feature 8..589 /label=immediate early CMV promoter /ApEinfo_fwdcolor=#ff1928 /ApEinfo_revcolor=#ff1928 misc_feature 583..583 /label=CMV transcription start (+1) /ApEinfo_fwdcolor=#ff19ff /ApEinfo_revcolor=#ff19ff misc_feature 2146..2865 /label=EGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 CDS 4096..4890 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=neomycin phosphotransferase /ApEinfo_fwdcolor=DarkOrange1 /ApEinfo_revcolor=DarkOrange1 misc_feature 3116..3571 /label=f1 single strand DNA origin /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3912..4047 /label=SV40 origin of replication /ApEinfo_fwdcolor=#eb3c34 /ApEinfo_revcolor=#eb3c34 misc_feature 4013..4013 /label=major transcription start /ApEinfo_fwdcolor=#ff41ff /ApEinfo_revcolor=#ff41ff misc_feature 3745..3816 /label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff2649 /ApEinfo_revcolor=#ff2649 misc_feature 3935..3955 /label=21-bp repeat /ApEinfo_fwdcolor=#ffab80 /ApEinfo_revcolor=#ffab80 misc_feature 1375..2127 /label=NDST2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 3057..3069 /label=mRNA ends somewhere here /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3668..3668 /label=transcription start (+1) of Kan/Neo resistance /ApEinfo_fwdcolor=#ff4e54 /ApEinfo_revcolor=#ff4e54 misc_feature 4008..4008 /label=major transcription start(1) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff33ff /ApEinfo_revcolor=#ff33ff misc_feature 5475..6118 /label=pUC plasmid replication origin /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 3913..3933 /label=21-bp repeat(1) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff96a6 /ApEinfo_revcolor=#ff96a6 misc_feature 3019..3024 /label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3633..3638 /label=-35 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3964..3964 /label=major transcription start(2) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff21ff /ApEinfo_revcolor=#ff21ff misc_feature 5126..5131 /label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3817..3888 /label=enhancer (72-bp tandem repeat)(1) /ApEinfo_label=enhancer (72-bp tandem repeat) /ApEinfo_fwdcolor=#ff5059 /ApEinfo_revcolor=#ff5059 misc_feature 3048..3053 /label=SV40 early polyA signal(1) /ApEinfo_label=SV40 early polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3656..3661 /label=-10 region /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 4002..4002 /label=major transcription start(3) /ApEinfo_label=major transcription start /ApEinfo_fwdcolor=#ff36ff /ApEinfo_revcolor=#ff36ff misc_feature 5139..5144 /label=HSV polyA signal(1) /ApEinfo_label=HSV polyA signal /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 3892..3912 /label=21-bp repeat(2) /ApEinfo_label=21-bp repeat /ApEinfo_fwdcolor=#ff7890 /ApEinfo_revcolor=#ff7890 misc_feature 652..1311 /label=CAT /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTAGCTTC GACGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 661 AAAATCACTG GATATACCAC CGTTGATATA TCCCAATCGC ATCGTAAAGA ACATTTTGAG 721 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 781 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 841 GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG 901 ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA 961 TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT 1021 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 1081 TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 1141 GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG 1201 CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA 1261 ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA 1321 GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AAGAGCTCAA GCTTTGCCAC 1381 CCAGCGTGGA GGACGGGGTC CACCCTGGTG GGCTGAGCGC GAGGCCGGAG GGCTGGGAGG 1441 CTGCGGCTGG GCTGAGCACT GCGGAGGGCC GTCGGCTTAT CCCAGGTCCC GGTCACGCAG 1501 CGGCCACGCT CGGGGAGCGC GCGGACCTGA GCGGGCACCA CGAGCCGCAG CGCGCCGCCT 1561 CCCCCTGGTG GGAGCGGTAC TTGGGCCCGA CTGCGGCGGA GCGTTCGCTC GGAGATGGCG 1621 GAGCCGCCGC GACGCGGCCC GAGGCCCCGC GAGCAGGGAA GCCGGGCAGC CCCGGGACGC 1681 GGCAGGGCCC GGGGACAGCG GGGACGCCGC CCACTCCACC GGGTATGAAG CTAAAATACA 1741 GTGGTTACAA CACAGGAGGT TGACAGGCCT GACCTTTGAA TCCCAGTTCA TCCACAAACG 1801 GAGCTTTGTA ATCTCGGATT GTAAGGGCCA AGCTTGTGGA CTTTACTGAC TTCTGATCTA 1861 AGAACCTCCA CCAGGGCTGA GGGAGCTCAA GTTTCAGCCT GAGGCATGGG CCAGGAGGCC 1921 ATGGTGACCT GAGACCCGTG GATTCTGTCC AGTACACCCT GTTGCTCCTT CTGCCTCCCC 1981 TCTCCTGTAT TGAAAGCGTT TGTGTTCATC TTATCAGGGA CCTGACCCTA AGAGAATCCT 2041 TcCCCTGCCA AGGCAGTTCC AGCCTcCAGA ACTCATCACC CCATCTCCTG AGGTGGTTCC 2101 TATACTCCCT CTCTTCTCCT CGCCATGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC 2161 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 2221 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 2281 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 2341 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 2401 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC 2461 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 2521 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 2581 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC 2641 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG 2701 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG 2761 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 2821 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT 2881 AGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC 2941 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG 3001 CAGCTTATAA TGGTTACAaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 3061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 3121 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 3181 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 3241 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 3301 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 3361 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 3421 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 3481 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 3541 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 3601 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 3661 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 3721 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 3781 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 3841 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 3901 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 3961 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 4021 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 4081 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 4141 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 4201 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 4261 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 4321 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 4381 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 4441 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 4501 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 4561 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 4621 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 4681 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 4741 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 4801 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 4861 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 4921 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 4981 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 5041 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 5101 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 5161 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 5221 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 5281 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 5341 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 5401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 5461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 5881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 5941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 6001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 6181 accgtattac cgccatgcat //