The abbreviated name of this ORF/gene: FLuc-fusion
The description of the protein encoded in this ORF: Firefly luciferase with 5 additional N-terminal aminoacid residues.
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 2263-3930
Authentic RNA pol II promoter was confirmed between bases +280 and +640 relative to the main start site of
transcription. Comment from J. Jimenez: Our original studies of the LEF1 5'UTR were based on characterizing
a third promoter in the 5'UTR that is responsible for generating a 3.0 kb transcript. Because we had evidence
of an authentic promoter, we performed N. blot analysis. We did also attempt RT-PCR, but had technical
problems due to the high GC content of the 5'UTR.
Possibility of aberrant splicing of the plasmids with LEF-1 was therefore ruled out only by the Northern-blot
technique (Fig. 2C and 3B).