IRESite_Id:573 summary record describing characteristics of eIF4GII IRES

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `573`	Version: `6`
Originaly submitted by: Martin Mokrejš	Submission date: `2009-03-22 18:09:07`
Reviewed by: Martin Mokrejš	Last change: `2009-10-13 13:22:14`

IRESite record type:

natural_transcript

The shape of the nucleic acid molecule translated: `linear`	The quality of the mRNA/+RNA sequence: `hopefully_full-length_mRNA`
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule: `eIF4GII`	The genetic origin of this natural mRNA/+RNA: `nuclear`

The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
9967556

Synonyms of the gene name:

Synonym: eIF4G2

The mRNA/+RNA description:

Homo sapiens eIF4GII mRNA, complete cds. Poly(A)-tail sequence was dropped.

The mRNA/+RNA sequence represented in the +DNA notation:

CAATCCCACA GAGTATTGAT GAGGAAACTG AAGTTTGGAG CGATCACATC ATTTTCCCAA GGTAACACAA GTGGCAAGAC AGCCGGGAAC CCCTACCCCA 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

TCCCCTTATT CAGCACATGA AATAAACAAG GGGCATCCAA ATCTTGCGGC AACGCCCCCG GGACATGCAT CGTCCCCTGG ACTCTCTCAA ACCCCTTATC 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CCTCTGGACA GAATGCAGGT CCAACCACGC TGGTATACCC TCAAACCCCT CAGACAATGA ATTCACAACC TCAAACCCGT TCTCCGTTTT TCCAGAGGCC 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

TCAAATACAG CCTCCTAGAG CTACCATCCC GAACAGCAGT CCTTCCATTC GTCCTGGTGC ACAGACACCC ACTGCAGTGT ACCAGGCTAA TCAGCACATC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ATGATGGTTA ACCATCTGCC CATGCCGTAC CCAGTGCCCC AGGGGCCTCA GTACTGTATA CCACAGTACC GTCATAGTGG CCCTCCTTAT GTTGGGCCCC 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CCCAACAATA TCCAGTTCAA CCACCGGGGC CAGGTCCTTT TTATCCTGGA CCAGGACCTG GGGACTTCCC CAATGCTTAT GGAACGCCTT TTTACCCAAG 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TCAGCCGGTG TATCAGTCAG CACCTATCAT AGTGCCTACG CAGCAACAGC CGCCTCCAGC CAAGAGAGAG AAAAAAACTA TAAGAATTCG GGATCCAAAC 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

CAGGGAGGTA AAGACATAAC AGAGGAGATT ATGTCTGGAG GTGGCAGCAG AAATCCTACT CCACCCATAG GAAGACCCAC GTCCACACCT ACTCCTCCTC 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

AGCAGCTGCC CAGCCAGGTC CCCGAGCACA GCCCTGTGGT TTATGGGACT GTGGAGAGCG CTCATCTTGC TGCCAGCACC CCTGTCACTG CAGCTAGCGA 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CCAGAAGCAA GAGGAGAAGC CAAAACCAGA TCCAGTGTTA AAGTCTCCTT CCCCAGTCCT TAGGCTAGTC CTCAGTGGAG AGAAGAAAGA ACAAGAAGGC 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

CAGACATCTG AAACTACTGC AATAGTATCC ATAGCAGAGC TTCCTCTGCC TCCATCACCT ACCACTGTTT CTTCTGTTGC TCGAAGTACA ATTGCAGCCC 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

CCACCTCTTC TGCTCTTAGT AGCCAACCAA TATTCACCAC TGCTATAGAT GACAGATGTG AACTCTCATC CCCAAGAGAA GACACAATTC CTATACCCAG 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

CCTCACATCT TGCACAGAAA CATCAGACCC TTTACCAACA AATGAAAATG ATGATGATAT ATGCAAGAAA CCCTGTAGTG TAGCACCTAA TGATATTCCA 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

CTGGTTTCTA GTACTAACCT AATTAATGAA ATAAATGGAG TTAGCGAAAA ATTATCAGCC ACGGAGAGCA TTGTGGAAAT AGTAAAACAG GAAGTATTGC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

CATTGACTCT TGAATTGGAG ATTCTCGAAA ATCCCCCAGA AGAAATGAAA CTGGAGTGTA TCCCAGCTCC CATCACCCCT TCCACAGTTC CTTCCTTTCC 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TCCAACTCCT CCAACTCCTC CAGCTTCTCC TCCTCACACT CCAGTCATTG TTCCTGCTGC TGCCACTACT GTTAGTTCTC CGAGTGCTGC CATCACAGTC 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CAGAGAGTCC TAGAGGAGGA CGAGAGCATA AGAACTTGCC TTAGTGAAGA TGCAAAAGAG ATTCAGAACA AAATAGAGGT AGAAGCAGAT GGGCAAACAG 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

AAGAGATTTT GGATTCTCAA AACTTAAATT CAAGAAGGAG CCCTGTCCCA GCTCAAATAG CTATAACTGT ACCAAAGACA TGGAAGAAAC CAAAAGATCG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

GACCCGAACC ACTGAAGAGA TGTTAGAGGC AGAATTGGAG CTTAAAGCTG AAGAGGAGCT TTCCATTGAC AAAGTACTTG AATCTGAACA AGATAAAATG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

AGCCAGGGGT TTCATCCTGA AAGAGACCCC TCTGACCTAA AAAAAGTGAA AGCTGTGGAA GAAAATGGAG AAGAAGCTGA GCCAGTACGT AATGGTGCTG 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

AGAGTGTTTC TGAGGGTGAA GGAATAGATG CTAATTCAGG CTCCACAGAT AGTTCTGGTG ATGGGGTTAC ATTTCCATTT AAACCAGAAT CCTGGAAGCC 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TACTGATACT GAAGGTAAGA AGCAGTATGA CAGGGAGTTT CTGCTGGACT TCCAGTTCAT GCCTGCCTGT ATACAAAAAC CAGAGGGCCT GCCTCCTATC 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

AGTGATGTGG TTCTTGACAA GATCAACCAA CCCAAATTGC CAATGCGAAC TCTGGATCCT CGAATTTTGC CTCGAGGACC AGACTTTACA CCAGCCTTTG 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

CTGATTTTGG AAGGCAGACA CCTGGTGGAA GAGGCGTACC TTTGTTGAAT GTTGGGTCAC GAAGATCTCA ACCTGGCCAA AGAAGAGAAC CCAGAAAGAT 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

CATCACAGTT TCTGTAAAAG AAGATGTACA CCTGAAAAAG GCAGAAAATG CCTGGAAGCC AAGCCAAAAA CGAGACAGCC AAGCCGATGA TCCCGAAAAC 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ATTAAAACCC AGGAGCTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG ATGTTCAATC AACTGATGAA GCAAGTGTCA GGACTTACTG 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

TTGACACAGA GGAGCGGCTG AAAGGAGTTA TTGACCTGGT CTTTGAGAAG GCTATTGATG AACCCAGTTT CTCTGTGGCT TACGCAAACA TGTGTCGATG 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT AACACAGTGA ATTTCCGGAA GCTGCTACTG AACCGTTGCC AGAAGGAGTT TGAAAAAGAT 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

AAAGCAGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TTGAGGCTGC CAGTGCTCCA GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCCA 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

AGGACAAAGC CCGGCGGAGA TCCATTGGCA ACATCAAGTT TATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC ATCATGCATG ACTGTGTGGT 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

GAAGCTGCTA AAGAACCATG ATGAAGAATC CCTGGAGTGC CTGTGTCGCC TGCTCACCAC CATTGGCAAA GACTTGGACT TTGAAAAAGC AAAGCCACGT 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

ATGGACCAGT ACTTTAATCA GATGGAGAAA ATTGTGAAAG AAAGAAAAAC CTCATCTAGG ATTCGGTTCA TGCTTCAAGA TGTTATAGAC CTAAGGCTGT 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

GCAATTGGGT ATCTCGAAGA GCAGATCAAG GGCCTAAAAC TATCGAACAG ATTCACAAAG AGGCTAAAAT AGAAGAACAA GAAGAGCAAA GGAAGGTCCA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

GCAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG AGAGTGGACG AAGGTGGGTG GAACACTGTA CAAGGGGCCA AGAACAGTCG GGTACTGGAC 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

CCCTCAAAAT TCCTAAAAAT CACTAAGCCT ACAATTGATG AAAAAATTCA GCTGGTACCT AAAGCACAGC TAGGCAGCTG GGGAAAAGGC AGCAGTGGTG 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

GAGCAAAGGC AAGTGAGACT GATGCCTTAC GGTCAAGTGC TTCCAGTTTA AACAGATTCT CTGCCCTGCA ACCTCCAGCA CCCTCAGGGT CCACGCCATC 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

CACGCCTGTA GAGTTTGATT CCCGAAGGAC CTTAACTAGT CGTGGAAGTA TGGGCAGGGA GAAGAATGAC AAGCCCCTTC CATCTGCAAC AGCTCGGCCA 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

AATACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGCTAG ACAATCAGTC TCAAGAAGAG CAGCGGAGAG AGATGCTGGA GACCGTGAAG CAGCTCACAG 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

GAGGTGTGGA TGTGGAGAGG AACAGCACTG AGGCTGAGCG AAATAAAACA AGGGAGTCAG CAAAACCAGA AATTTCAGCA ATGTCAGCTC ATGACAAGGC 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

TGCATTATCA GAAGAGGAAC TGGAGAGGAA GTCGAAATCT ATCATTGATG AATTTCTACA CATTAATGAT TTTAAGGAAG CCATGCAGTG TGTGGAAGAG 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

CTGAATGCCC AGGGCCTACT ACATGTTTTT GTGAGAGTGG GAGTGGAGTC CACCCTGGAA AGGAGCCAGA TCACCAGGGA TCACATGGGC CAACTACTCT 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

ATCAGCTGGT ACAGTCAGAA AAACTCAGCA AACAGGACTT TTTCAAAGGT TTTTCAGAAA CTTTGGAATT GGCAGATGAC ATGGCCATTG ATATTCCCCA 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

TATTTGGTTG TACCTTGCTG AACTGGTGAC CCCCATGTTA AAAGAAGGTG GAATCTCCAT GAGAGAACTT ACCATAGAAT TTAGCAAACC TTTACTTCCT 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

GTTGGAAGAG CTGGGGTCTT GCTATCTGAA ATATTGCACC TACTATGCAA ACAAATGAGC CATAAGAAAG TGGGAGCCTT ATGGAGGGAG GCTGACCTCA 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

GCTGGAAGGA CTTTTTACCA GAAGGAGAAG ATGTACATAA TTTTCTTTTG GAGCAGAAGT TGGACTTCAT AGAGTCTGAC AGTCCCTGTT CCTCTGAAGC 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

ACTTTCAAAG AAAGAACTGT CTGCCGAAGA GCTGTATAAG CGACTCGAGA AACTCATTAT TGAGGACAAA GCGAATGATG AACAGATCTT TGACTGGGTA 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

GAGGCTAATC TAGACGAAAT CCAGATGAGT TCACCTACAT TCCTTAGAGC TTTAATGACT GCTGTTTGTA AAGCAGCTAT TATAGCCGAC TCTTCTACCT 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

TCAGAGTGGA CACTGCTGTT ATCAAGCAGA GAGTGCCGAT CTTACTCAAG TACCTAGACT CAGATACAGA GAAGGAACTG CAAGCACTTT ATGCACTACA 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

AGCATCGATA GTAAAACTTG ATCAACCTGC CAATTTGCTG CGGATGTTTT TTGATTGTCT ATATGACGAG GAGGTGATCT CCGAGGATGC CTTCTACAAA 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

TGGGAGAGCA GCAAGGACCC TGCAGAGCAG AATGGGAAGG GCGTGGCTCT GAAATCTGTC ACGGCATTCT TCACGTGGCT GCGGGAAGCA GAAGAGGAGT 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

CTGAGGATAA CTAAAACTTC AAATACACAA AATGAAACAA AAGAAACAAT TTAAGTATTT TTTTAAAAAG TTTCACGTCT TCGCCAATCA CAGTGCAGCA 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

AGGCCAATTC TCGCAGAAAC CCCACGTGTG CACGAGTGGG AGAGGGGAAA GAGAAAAAAA GGTGTACATG GAGGAAAAAG GTACTGGATA AAAGTAAACT 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

TCAAACCTTA GGGCGGGAGC ACTAAAACCA AAATACATGT ATTATTTATA GAAAATATTT TCTGTTTTAA TCTTTTCTTT TTAAACAAGG ACTCATACTT 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

AAAAAAATGT TTAGCAAAAA AAAAAAAAAG TTGAGAACTT TTAATTTATT TTAAGGACTG CAAATGCCAG TGTAATTTTT TAATTTGCAG TTTCTGTAAA 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

CAACTGTATA ATAGAAAAGC AGAGAAATAA ATTTCCCTCC CCTTCAAGAT GCACCTCATG TTTGTTTTAA GGTATAGCAT TTAGTCCAGA TTTGAGAAAG 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

TTTGGGGTGA ACAAGGTAAG AAAGATTTTT TTTTTTTTGG CATCAAATCT TTCTGCCTGC CTCTCAGCTT GCTTCAGAAA ATTTAAAAAA TCACAATAGT 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

AATCAAAACA TACATAACAT TGAAACAGAA GGAAATGCTG TGGACCACAG AACTCCAAGA ATTGTTTAAA AAAAAAAAAG TGCTACCCTG AGAAAAGTAC 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

TCTTAATATC TCTTGAAATC TTTAGAGCAA CTTTAAGGCT TGTAAATACA TAGAACAAAT ATTTAAAAAA ACAAAAAGAA ATTGACTCAG TACTATTTCT 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

TTTCACTTTG AAAATATAAA GAACAAAATA AAGACAAACA TTGCAAGTTT
^5801      ^5811      ^5821      ^5831      ^5841

Credibility of mRNA sequence:

end-to-end_sequence_reverse_engineered_and_should_match_experiment

The organism containing this mRNA with IRES segment in its genome:

Homo sapiens

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
1

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
eIF4GII

The description of the protein encoded in this ORF:
eukaryotic translation initiation factor 4G, isoform II

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

257-5014

Remarks:

This is the sequence reported by Gradi et al. (1998) in GI:9967556 alias  and is different from the
eIF4GII-long variant reported later by Johannes and Sarnow (1998) and stored under IRESiteID:574. Please note
the trailing ".2" in the previously mentioned GenBank accession number which means there was a revision of the
primary sequence under that accession number in the meantime since its initial submission. There is no
well-matching sequence in RefSeq to AF012072.2, the closest match would be NM_003760.3 but the first three
exons of NM_003760.3 (1-126, 127-310, 311-386) are replaced by 60bp of some other origin in AF012072.2.


>NC_000001 Homo sapiens chromosome 1, GRCh37 primary reference assembly.; LEN=370369
>AF012072.2|IRESite_Id:573 (this entry) Homo sapiens eIF4GII mRNA, complete cds.; LEN=5880

65465-65525  (1-61)   100% -> (GT/AG) 24
and continue with shared exons (only the first listed below)
125854-125982  (62-190)   100% -> (GT/AG) 24
[cut]

>NC_000001 Homo sapiens chromosome 1, GRCh37 primary reference assembly.; LEN=370369
>NM_003760.3 Homo sapiens eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), mRNA.; LEN=6235

1-126  (31-156)   100% -> (GT/AG) 24
490-673  (157-340)   100% -> (GT/AG) 24
87635-87710  (341-416)   100% -> (GT/AG) 24
and continue with shared exons (only the first listed below)
125854-125982  (417-545)   100% -> (GT/AG) 24
[cut]

Our extended mRNA sequence using ESTs in IRESite_Id:574 spans in front of the genomic contig NC_000001:

>NC_000001 Homo sapiens chromosome 1, GRCh37 primary reference assembly.; LEN=370369
>IRESite_Id:574 eIF4GII-long (chromosome1), IRESite extension of NM_003760.3 by 30 bp; LEN=6205

1-126  (31-156)                100% -> (GT/AG) 24   (126bp exon)
490-673  (157-340)             100% -> (GT/AG) 24   (184bp exon)
87635-87710  (341-416)         100% -> (GT/AG) 24   (76bp exon)
125854-125982  (417-545)       100% -> (GT/AG) 24   (129bp exon)


Here is conformation against whole chromosome 1 assembly:

>NT_004610 Homo sapiens chromosome 1 genomic contig, GRCh37 reference primary assembly.; LEN=16558170
>IRESite_Id:574 eIF4GII-long (chromosome1); LEN=6205

(complement)

[cut]
8057447-8057575  (5661-5789)   100% <- (GT/AG) 24   (129bp exon)
8095719-8095794  (5790-5865)   100% <- (GT/AG) 24   (76 bp exon)
8182756-8182939  (5866-6049)   100% <- (GT/AG) 24   (184bp exon)
8183303-8183458  (6050-6205)   100%                 (156bp exon)



For comparison of the eIF4G (IRESiteID:548), eIF4GI, eIF4GI-ext(IRESiteID:576), this eIF4GII and
eIF4GII-long(IRESiteID:574) see Figure 6 in Johannes and Sarnow (1998).

Citations:

Gradi A., Imataka H., Svitkin Y. V., Rom E., Raught B., Morino S., Sonenberg N. (1998) A novel functional human eukaryotic translation initiation factor 4G. Mol. Cell. Biol. 18(1):334-342

IRESs:

IRES:

Version: `7`	Last change: `2009-10-13 13:40:28`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `eIF4GII`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

1-256

Conclusion:
never_claimed_to_be_IRES

How IRES boundaries were determined:
guessed

The sequence of IRES region aligned to its secondary structure (if available):

CAATCCCACA GAGTATTGAT GAGGAAACTG AAGTTTGGAG CGATCACATC ATTTTCCCAA GGTAACACAA GTGGCAAGAC AGCCGGGAAC CCCTACCCCA 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

TCCCCTTATT CAGCACATGA AATAAACAAG GGGCATCCAA ATCTTGCGGC AACGCCCCCG GGACATGCAT CGTCCCCTGG ACTCTCTCAA ACCCCTTATC 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

CCTCTGGACA GAATGCAGGT CCAACCACGC TGGTATACCC TCAAACCCCT CAGACA
^201       ^211       ^221       ^231       ^241       ^251

Remarks:

Nobody tried. Anyway, the eIF4GII mRNA overlaps partly with the eIF4GII-long (IRESite_Id:574) studied for IRES
activity and found not to have it (see Figure 8 in Johannes and Sarnow, 1998). See Figure 6D in their article
for comparison of the 5'-UTR sequences of both chromosome 1 variants.

Citations:

Johannes G., Sarnow P. (1998) Cap-independent polysomal association of natural mRNAs encoding c-myc, BiP, and eIF4G conferred by internal ribosome entry sites. RNA. 4(12):1500-1513

Last change to the database: 2019-03-18 09:32:49 GMT+1